Pewarnaan BTA

Pewarnaan BTA

Tujuan:

Diagnosis (terutama TB, infeksi mycobacterium lain, dan kadang positif pada

beberapa actinomyces)

Sampel:

Sputum, swab, pus, urine, darah, feces, liquor, cairan pleura, ascites/cairan

peritoneal.

Reagen:

1. Carbol fuchsin :

- Basic fuchsin                                           3,0 gr

- Etanol/methanol                                    100 ml

- Kristal phenol                                          45 gr

- Aquadest                                               900 ml

2. larutan dekolorisasi :

- HCl pekat                                                30 ml

- Etanol                                                    970 ml

3. pewarna kontras :

- Methylen blue khlorida                           30 ml

- Air suling/aquadest                              1000 ml

Interpretasi:

§ BTA (+): basil berwarna merah

§ BTA (-): basil berwarna biru